FastBreakā¢ Cell Lysis Reagent
Quick <i>E. coli</i> Cell Culture Lysis, No Centrifugation or Mechanical Disruption Required
- Eliminate centrifugation and mechanical disruption
- Simple, add-and-incubate protocol
- Compatible with manual or robotic platforms
Catalog Number:
Size
Catalog Number: V8571
Catalog Number: V8572
Catalog Number: V8573
Efficient Lysis of E. coli Cultures; No Centrifugation or Mechanical Disruption Needed
FastBreakā¢ Cell Lysis Reagent is provided as a 10X concentrate and contains a proprietary nonionic detergent to facilitate lysis. Add the reagent directly to E. coli cultures. Following a brief incubation, the cells are disrupted, and the protein Ā released. Recombinant proteins can be directly screened in the cell extract or purified by the addition of the appropriate affinity matrix such as the MagneHisā¢ Protein Purification System. This product is suitable for both manual and automated protocols.Ā
Eliminate centrifugation and mechanical disruption with this easy-to-use, add and incubate reagent, compatible with manual and robotic platforms.
Manual purification of a 6X His-tagged fusion protein using FastBreakā¢ Cell Lysis Reagent and the MagneHisā¢ Protein Purification System. Lane 1, bacterial cell lysate expressing 6X His-tagged firefly luciferase lysed by the addition of FastBreakā¢ Reagent; lane 2, postlysis cell supernatant demonstrating that the protein is solubilized; lane 3, flowthrough of cell lysate after the addition of MagneHisā¢ Ni-Particles; lane 4, eluted 6X His-tagged firefly luciferase; lane 5, Broad Range Protein Molecular Weight Markers (Cat.# V8491).
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
FastBreakā¢ Cell Lysis Reagent, 10X |
V857A | 1 × 15ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
This product is licensed for use under U.S. Pat. No. 6,174,704.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
FastBreakā¢ Cell Lysis Reagent, 10X |
V857B | 1 × 60ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
This product is licensed for use under U.S. Pat. No. 6,174,704.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
FastBreakā¢ Cell Lysis Reagent, 10X |
V857C | 1 × 100ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
This product is licensed for use under U.S. Pat. No. 6,174,704.
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MagneHisā¢ Protein Purification System
Purification of His- or HQ-tagged, proteins from a crude cell lysate on paramagnetic particles with either a manual (requires use of a magnetic stand) or automated procedure.
V8500, V8550
Broad Range Protein Molecular Weight Markers
Nine protein bands at convenient molecular weights of 10, 15, 25, 35, 50, 75, 100, 150 and 225kDa.
V8491
