Maxwell® RSC Fecal Microbiome DNA Kit
Reliable, Automated DNA Purification from Fecal Samples
- Purifies high-quality DNA from ≤300mg of feces
- Ready for use in PCR, qPCR, 16S metagenomic sequencing
- No need for labor-intensive sample processing
Catalog Number:
Size
Catalog Number: AS1700
Simple, Reproducible DNA Extraction from Fecal Samples
The Maxwell® RSC Fecal Microbiome DNA Kit used with the Maxwell® RSC Instruments provides a simple method for efficient, automated isolation of nucleic acid from human and mammalian fecal samples.
Fecal microbiome characterization is a rapidly evolving field, with continued development of downstream assays. Complex analyses such as next-generation sequencing can enhance data quality by measuring the abundance of various bacterial species. Many extraction methods for fecal microbiome DNA involve several manual processing steps and mechanical disruption. The Maxwell® RSC Fecal Microbiome DNA Kit purifies microbiome DNA for characterization in downstream assays including qPCR and next-generation sequencing.
Advantages
- Simple, automated protocol requires minimal hands-on steps.
- No organic extractions or laborious sample preprocessing steps.
- Optimized to extract microbial DNA from fecal samples for metagenomic analysis.
A quick protocol for fecal DNA extraction without the need for bead beating.
Yield and Concentration of DNA from Fecal Samples
DNA from 100mg and 300mg of human fecal samples was extracted using the Maxwell® RSC Fecal Microbiome DNA Kit. Yield and concentration of extracted DNA was determined using the QuantiFluor® dsDNA System (Cat.# E2670, E2671).
Maxwell® Fecal Microbiome Kit: Compatibility with Stool Collection Devices
We continue to test the performance of the Maxwell® RSC Fecal Microbiome DNA Purification Kit for isolation of DNA under various conditions. View the application notes below to see protocols and example NGS data demonstrating use of this kit with different collection devices, including Copan FecalSwab® DNA/RNA Shield, OMNIgene• GUT and S.T. A. R. Buffer.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
Maxwell® RSC Cartridge (RSCJ) |
AS159A | 48 × 1 each | ||
|
Proteinase K (PK) Solution |
MC500C | 2 × 1ml | View Product | |
Lysis Buffer |
MC141A | 1 × 100ml | View Product | |
|
RNase A Solution |
A797C | 1 × 1ml | 4mg/ml | View Product |
|
Elution Buffer |
A828D | 1 × 20ml | View Product | |
Binding Buffer |
MC501D | 1 × 20ml | ||
RSC Plungers |
AS1670 | 1 × 48/pack | View Product | |
Elution Tubes (0.5ml) |
AS620A | 1 × 50/pk | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Software and Firmware
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