Notes: In this paper, researchers demonstrated that staurosporine-induced calpastatin degradation is prevented by the pan-caspase inhibitor Z-VAD-FMK. Stably transfected SH-SY5Y cells expressing calpastatin were pretreated with 50uM Z-VAD-FMK for an undisclosed amount of time before treatment with 0-0.5 µM staurosporine for 6 hours. Cell lysates were then analyzed by Western blotting to demonstrate the presence of caspase activity. (2668)

Notes: The caspase Inhibitor Z-VAD-FMK was added to human umbilical vascular endothelial cell (VEC) cultures to examine its effect on proliferation when cells were subjected to an electric field. When added to cultures Z-VAD-FMK had no effect on proliferation of VECs.  (2827)

Notes: Spontaneously immortalized granulosa cells were incubated with 50μM of the pan-caspase inhibitor Z-VAD-FMK to demonstrate that apoptosis occurs after serum withdrawal or EGTA treatment. The inhibitor was added after treatment of the cells for 5 hours. Researchers also used Promega's Colorimetric CaspACE™ Assay System to measure caspase-3 activity in cell lysates. Results were expressed as fold increase in activity.  (2669)