Promega Corporation

Improved Response Ratio for NFκB Inhibition Analysis Using New Luciferase Reporter...

Improved Response Ratio for NFκB Inhibition Analysis Using New Luciferase Reporter Vector (pGL4-32[luc2PNFκB-REHygro])

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Abstract

Promega has developed a new luciferase reporter vector for single- or dual-reporter gene measurements. Our experiments were performed in order to inhibit TNFα-induced nuclear transcription factor kappa B (NF-κB) activity by a natural product. Results with this new vector show improved reporter expression of firefly luciferase luminescence compared to a competitor vector. This new vector allows more accurate and precise measurement of NF-κB inhibition by bioactive compounds paralleled by an economically better consumption of the firefly luciferase vector.

Cell Notes 21, 18–19.

Marc Schumacher, Mario Dicato and Marc Diederich*

Laboratorie de Biologie Moléculaire et Cellulaire du Cancer, Hôpital Kirchberg Luxembourg, Luxemborg, *corresponding author
Publication Date: 2008

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