UGT-Glo™ Assay Technical Bulletin

Literature # TB551

The UGT-Glo™ Assay provides a luminescent method for measuring UDP glucuronosyltransferase (UGT) activity. The UGT family of enzymes are involved in the metabolism of various compounds in the body. These enzymes transfer a hydrophilic glucuronic acid moiety to their substrates, rendering them more water soluble and suitable for excretion. The UGT-Glo™ Assay is designed to measure UGT activity from a variety of sources, such as microsomes containing recombinantly expressed enzymes or microsomal preparations derived from mammalian tissues, and to test the effect of various chemicals on UGT activity. The assay uses proluciferin substrates for UGT enzymes. These substrates are first modified by UGT enzymes, then converted to the luciferin derivatives using D-Cysteine during the detection step. Without conversion to a luciferin derivative by D-Cysteine, the proluciferin substrates will not produce light when incubated with luciferase.