Terminal Deoxynucleotidyl Transferase, Recombinant Protocol
Literature # 9PIM187
This enzyme catalyzes the repetitive addition of mononucleotides to the terminal 3′-OH of a DNA initiator, accompanied by the release of inorganic phosphate. Single-stranded DNA is preferred as an initiator. Polymerization is not template-dependent. The addition of 1mM Co2+ (as CoCl2) in the reaction buffer allows the tailing of 3′-ends with varying degrees of efficiency.
Printed in USA. Revised 4/07.