Reporter Vectors

NanoLuc® Luciferase Reporters

State-of-the-art reporter technology offering unrivaled sensitivity and versatility in a very small luciferase. Find the pNL reporter that is right for your application.

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pGL4 Luciferase Reporter Vectors

Optimized luciferase reporter vectors with both firefly and Renilla luciferases in a variety of configurations. Now also available with response elements to study pathways of interest.

• pGL4 Vector Product Listing

miRNA Target Expression Vector

The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3´ of the firefly luciferase gene (luc2).

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pRL Renilla Luciferase Control Vectors

Provide constitutive expression of Renilla luciferase. Expression of Renilla luciferase provides an internal control value to which expression of the experimental firefly luciferase reporter gene may be normalized.

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This animation introduces the concept of reporter gene assays, using the Dual-Luciferase® Assay as a model.

This chapter of the Protocols and Applications Guide discusses the use of bioluminescent reporters to study eukarytoic gene expression and regulation including the use of single-reporter assays, dual-reporter assays, and live-cell substrates.