Designed to meet CODIS and European standards, the PowerPlex® Fusion System enables laboratories to:
- Achieve the most inter database compatibility and highest discrimination of any autosomal STR kit.
- Improve laboratory efficiencies with rapid cycling and direct amplification protocols.
- Obtain a higher success rate with difficult casework samples due to robustness and sensitivity.
- Simplify validation and QC efforts by using one kit for both casework and databasing sections.
The PowerPlex® Fusion System provides all of the materials needed for co-amplification and five-color fluorescent detection of 24 loci (23 STR loci and Amelogenin), including the CODIS core loci and the European Standard Set (ESS) loci. With 24 loci, the system offers the most STR loci and highest discrimination from a single reaction and delivers more information in demanding forensic, paternity and relationship testing cases. Utilizing proven STR chemistries on existing instrument platforms and software, the PowerPlex® Fusion System requires no software or instrument upgrades.
Figure 1. Configuration of the PowerPlex® Fusion System. The PowerPlex® Fusion System allows co-amplification and four-color detection of 24 loci, including all CODIS and ESS loci: D3S1358, D1S1656, D2S441, D10S1248, D13S317, D16S539, D18S51, D2S1338, CSF1PO, TH01, vWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, FGA, D22S1045, plus Penta E, Penta D, DYS391 and Amelogenin.
The PowerPlex® Fusion System reliability produces complete STR profiles from as little as 100pg of human DNA.
Figure 2a. Amplification of 100pg of human DNA using 30 cycles and the PowerPlex® Fusion System. Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection). Figure 2b. The PowerPlex® Fusion System demonstrates superior sensitivity, generating full profiles with as little as 100pg input DNA.
Superior Inhibitor Tolerance
With a robust buffer system designed to tolerate the most challenging inhibitors, the PowerPlex® Fusion System minimizes the need to re-amplify samples generally thought to be too challenging to run and saves your lab countless hours of repeat analysis.
Figure 3. Human genomic DNA (500pg) was amplified with each listed kit in the presence of common PCR inhibitors. Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection). Percentage of alleles detected are indicated for each system.
Direct-Amplification Compatibility for Most Sample Types
Building on the proven flexibility of our PowerPlex® 21 System, the PowerPlex® Fusion System allows direct amplification from FTA® card punches as well as pretreated non FTA cards and commonly used swabs to streamline workflow and expand sample throughput.
Figure 4a. One 1.2mm buccal punch of an S&S903 card was pre treated with PunchSolution™ Reagent as recommended in the PunchSolution™ Kit Technical Manual TMD038. Figure 4b. A swab was pre treated with SwabSolution™ Reagent. Following incubation, 2µl of extract was added to the PCR amplification mix and amplified as described in the PowerPlex® Fusion System Technical Manual TMD039. Figure 4c. Direct amplification of one 1.2mm FTA® card punch containing blood using the protocol described in the PowerPlex® Fusion System Technical Manual TMD039. Figure 4d. Direct amplification of two 1.2mm FTA® card punches from a buccal sample using the protocol described in the PowerPlex® Fusion System Technical Manual TMD039. Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).
Fewer Adventitious Matches
As DNA databases continue to grow and international cooperation increases, the need for a common set of markers is required to facilitate data sharing and to reduce adventitious matches. The PowerPlex® Fusion System enables increased discriminatory power and data sharing possibilities by means of the incorporation of common and informative loci used throughout the world.
Figure 5 indicates the Probability of Identity values for a variety of currently available STR systems and standards. A significant increase in discriminatory power is seen with the PowerPlex® Fusion System over other well established STR systems.
Figure 5. A Probability of Identity value (PI) is defined as the probability that two individuals selected at random will have an identical genotype at the tested locus. It is calculated by summing the square of the genotype frequencies. The PI for PowerPlex® Fusion System is significantly lower than the PI values seen with currently available STR typing kits. Data were kindly provided by John Butler (NIST) using data collected by Carolyn Hill and analysis developed by Dave Duewer.